Instrumental Methods of Analysis Unit 1 Notes
UV Visible spectroscopy
Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
Instrumentation- Sources of radiation, wavelength selectors, sample cells, detectors Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
Applications- Spectrophotometric titrations, Single component and multi component analysis
Fluorimetry
Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications